# Shorthand notation for Sphingolipids (SP)

### From LipidomicsWiki

# Rules for the annotation

- The
**sphingoid backbone**is annotated by the**number of hydroxyl groups**in the sphingoid base (m for mono, d for di, t for tri) and separated by a slash from the**number of carbons:number of double bonds**of the N-linked fatty acid. Positions of hydroxyl groups and double bonds including geometry are indicated as described for fatty acyls (FA). - If the
**sphingoid base is not known**the sum of sphingoid base and fatty acid is shown as number of carbons:number of double bonds. Calculations are based on the number of hydroxyl groups of the**major sphingoid base for that organism**(dihydroxy in mammals). When the number of hydroxyl groups is known it is shown in front of the number of carbons (hydroxyl group level, e for tetra). - For further characterization of N-linked fatty acids, rules as described in section 3 apply. A fatty acid that is ester-bound to a N-linked ω-OH fatty acid is shown in square brackets as [ωFA C-atoms:double bonds].
- Sugar moieties:
- The identity of sugar is not known:
- Hex - Hexosyl
- Hex2 - DiHexosyl

- The identity of the sugar is proven experimentally :
- Gal - Galactosyl
- Glc – Glucosyl
- Lac - Lactosyl

- The identity of sugar is not known:

# Lipid class abbreviations

- Ceramide - Cer
- Ceramide-1-phosphate - C1P
- Sphingoid base - SPH
- Sphingoid base-1-phosphate - S1P
- Sphingomyelin - SM
- Hexosylceramide - HexCer
- Glucosylceramide - GlcCer
- Galactosylceramide - GalCer
- Dihexosylceramide – Hex2Cer
- Lactosylceramide - LacCer

# Examples

Bond type | Lipid class level mass$ | Hydroxyl group level | Fatty acyl level | LIPID MAPS Sphingoid base/Fatty acyl structure level |
---|---|---|---|---|

Cer (537) | Cer 34:1 | Cer d34:1 | Cer d18:1/16:0 | Cer d18:1(4E)(1OH,3OH)/16:0 |

Cer (539) | Cer 34:0 | Cer d34:0 | Cer d18:0/16:0 | Cer d18:0 (1OH,3OH)/16:0 |

C1P (617) | C1P 34:1 | C1P d34:1 | C1P d18:1/16:0 | C1P d18:1(4E)(1OH,3OH)/16:0 |

SM (702) | SM 34:1 | SM d34:1 | SM d18:1/16:0 | SM d18:1(4E)(1OH,3OH)/16:0 |

SM (704) | SM 34:0 | SM d34:0 | SM d18:0/16:0 | SM d18:0 (1OH,3OH)/16:0 |

SM (840) | SM 44:2 | SM d44:2 | SM d20:1/24:1 | SM d20:1(4E)(1OH,3OH)/24:1(15Z) |

HexCer (701) | HexCer 34:1 | HexCer d34:1 | HexCer d18:1/16:0 | GlcCer d18:1(4E)(1OH,3OH)/16:0 |

HexCer (701) | HexCer 34:1 | HexCer d34:1 | HexCer d18:1/16:0 | GalCer d18:1(4E)(1OH,3OH)/16:0 |

Hex2Cer (861) | Hex2Cer 34:1 | Hex2Cer d34:1 | Hex2Cer d18:1/16:0 | LacCer d18:1(4E)(1OH,3OH)/16:0 |

Sphingoid base | Lipid class level mass$ | Hydroxyl group level | LIPID MAPS Sphingoid base structure level |
---|---|---|---|

sphingosine | SPH (299) | SPH d18:1 | SPH d18:1(4E)(1OH,3OH) |

sphinganine | SPH (301) | SPH d18:0 | SPH d18:0(1OH,3OH) |

sphingadiene | SPH (297) | SPH d18:2 | SPH d18:2(4E,8E)(1OH,3OH) |

phytosphingosine | SPH (317) | SPH t18:0 | SPH t18:0(1OH,3OH,4OH) |

sphingosine–C20 | SPH (327) | SPH d20:1 | SPH d20:1(4E)(1OH,3OH) |

sphingosine | S1P (379) | S1P d18:1 | S1P d18:1(4E)(1OH,3OH) |

sphinganine | S1P (381) | S1P d18:0 | S1P d18:0(1OH,3OH) |

1-deoxymethyl-sphinganine | SPH (271) | SPH m17:0 | SPH m17:0(2OH) |

1-deoxy-sphinganine | SPH (285) | SPH m18:0 | SPH m18:0(3OH) |

$ m is the uncharged molecular mass

§ Annotation based on the assumption of a sphingoid base with two hydroxyl groups