Lipoprotein analysis by FPLC - ESI-MS/MS - Wiesner et al.

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== FPLC separation  ==
== FPLC separation  ==
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=== Pump  ===
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Lipoprotein fractions are prepared by FPLC separation as described in detail in [[Lipoprotein separation by FPLC - Wiesner et al.|Lipoprotein separation by FPLC - Wiesner et al.]]
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*Type:
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*Mode: isocratic or gradient
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*Solvent(s):
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*Gradient:
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{| border="1" class="wikitable" style="text-align: center;"
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|-
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! Time [min]
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! Flow [ml/min]
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!  % Solvent A
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!  % Solvent B
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|-
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! 0
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| 0.2
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| 100
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| 0
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|-
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! 1.0
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| 0.2
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| 50
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| 50
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|-
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! 1.5
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| 0.2
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| 100
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| 0
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|}
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=== Column  ===
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*Type:
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*Temperature:
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=== Fractionation  ===
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*Type:
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*Injection volume:
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*Wash solvent:
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<br>
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== Mass spectrometric analysis  ==
== Mass spectrometric analysis  ==
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*[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18832345 Wiesner et al. J Lipid Res. 2009. Epub ahead of print]
*[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18832345 Wiesner et al. J Lipid Res. 2009. Epub ahead of print]
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[[Category:Lipoprotein_analysis]] [[Category:FPLC]][[Category:Flow_injection_analysis]] [[Category:ESI]] [[Category:Triple_quadrupole]]
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[[Category:Lipoprotein_analysis]] [[Category:FPLC]] [[Category:Flow_injection_analysis]] [[Category:ESI]] [[Category:Triple_quadrupole]]

Revision as of 19:29, 2 February 2009

Contents

FPLC separation

Lipoprotein fractions are prepared by FPLC separation as described in detail in Lipoprotein separation by FPLC - Wiesner et al.

Mass spectrometric analysis

FPLC fractions are analyzed using the following mass spectrometric methods based on direct flow injection:


Method validation

Accuracy

Precision

Limit of detection

Recovery

Sample data

Mass spectra

Chromatogram

Reference

Retrieved from "https://www.lipidomicnet.org/index.php/Lipoprotein_analysis_by_FPLC_-_ESI-MS/MS_-_Wiesner_et_al."
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