Lipoprotein analysis by FPLC - ESI-MS/MS - Wiesner et al.

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(New page: == FPLC separation == === Pump === *Type: *Mode: isocratic or gradient *Solvent(s): *Gradient: {| border="1" class="wikitable" style="text-align: center;" |- ! Time [min] ! Flow ...)
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== FPLC separation  ==
== FPLC separation  ==
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=== Pump  ===
=== Pump  ===
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*Gradient:
*Gradient:
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{| border="1" style="text-align: center;" class="wikitable"
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! Time [min]  
! Time [min]  
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=== Column  ===
=== Column  ===
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if used
 
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*Type:  
*Temperature:
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=== Autosampler  ===
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=== Fractionation ===
*Type:  
*Type:  
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*Wash solvent:
*Wash solvent:
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=== Mass spectrometer ===
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== Mass spectrometric analysis ==
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[[Ceramide - ESI-MS/MS - Liebisch et al.]]
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*Type:
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*Ionization mode:
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*Ionization voltage:
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*Source temperature(s):
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*Collision gas:
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*Collision gas pressure:
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*MS/MS-conditions:
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**multiple reaction monitoring table
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{| border="1" class="wikitable" style="text-align: center;"
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|-
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! Analyte
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! Precursor [m/z]  
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! Precursor [m/z]  
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! Collision energy [eV]
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! species 1
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! species 2
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== Data analysis and quantification  ==
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=== Data handling  ===
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*chromatogram, spectrum and peak processing
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=== Isotope correction  ===
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*if necessary
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=== Calibration and quantification  ===
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*calibration type: matrix or external calibration by standard addition to sample matrix
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*species used for calibration
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! Species
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! Material 1
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! Material 2
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! Species 1
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| range pmol
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| range pmol
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! Species 1
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| range pmol
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| range pmol
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<br>
 
== Method validation  ==
== Method validation  ==
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*[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18832345 Wiesner et al. J Lipid Res. 2009. Epub ahead of print]
*[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18832345 Wiesner et al. J Lipid Res. 2009. Epub ahead of print]
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[[Category:Lipoprotein_analysis]]
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[[Category:Lipoprotein_analysis]] [[Category:Lipid_mass_spectrometry]]
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[[Category:Lipid_mass_spectrometry]]
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Revision as of 10:12, 27 January 2009

Contents

FPLC separation

Pump

  • Type:
  • Mode: isocratic or gradient
  • Solvent(s):
  • Gradient:
Time [min] Flow [ml/min]  % Solvent A  % Solvent B
0 0.2 100 0
1.0 0.2 50 50
1.5 0.2 100 0

Column

  • Type:
  • Temperature:

Fractionation

  • Type:
  • Injection volume:
  • Wash solvent:

Mass spectrometric analysis

Ceramide - ESI-MS/MS - Liebisch et al.


Method validation

Accuracy

Precision

Limit of detection

Recovery

Sample data

Mass spectra

Chromatogram

Reference

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