Lipoprotein analysis by FPLC - ESI-MS/MS - Wiesner et al.

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Revision as of 09:05, 27 January 2009

Contents

FPLC separation

Pump

  • Type:
  • Mode: isocratic or gradient
  • Solvent(s):
  • Gradient:
Time [min] Flow [ml/min]  % Solvent A  % Solvent B
0 0.2 100 0
1.0 0.2 50 50
1.5 0.2 100 0

Column

if used

  • Type:
  • Temperature:

Autosampler

  • Type:
  • Injection volume:
  • Wash solvent:

Mass spectrometer

  • Type:
  • Ionization mode:
  • Ionization voltage:
  • Source temperature(s):
  • Collision gas:
  • Collision gas pressure:
  • MS/MS-conditions:
    • multiple reaction monitoring table
Analyte Precursor [m/z] Precursor [m/z] Collision energy [eV]
species 1 m/z 1 m/z 2 x
species 2 m/z 1 m/z 2 x

Data analysis and quantification

Data handling

  • chromatogram, spectrum and peak processing

Isotope correction

  • if necessary

Calibration and quantification

  • calibration type: matrix or external calibration by standard addition to sample matrix
  • species used for calibration
Species Material 1 Material 2
Species 1 range pmol range pmol
Species 1 range pmol range pmol


Method validation

Accuracy

Precision

Limit of detection

Recovery

Sample data

Mass spectra

Chromatogram

Reference

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