Lipoprotein analysis by FPLC - ESI-MS/MS - Wiesner et al.
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(New page: == FPLC separation == === Pump === *Type: *Mode: isocratic or gradient *Solvent(s): *Gradient: {| border="1" class="wikitable" style="text-align: center;" |- ! Time [min] ! Flow ...)
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Revision as of 09:05, 27 January 2009
Contents |
FPLC separation
Pump
- Type:
- Mode: isocratic or gradient
- Solvent(s):
- Gradient:
Time [min] | Flow [ml/min] | % Solvent A | % Solvent B |
---|---|---|---|
0 | 0.2 | 100 | 0 |
1.0 | 0.2 | 50 | 50 |
1.5 | 0.2 | 100 | 0 |
Column
if used
- Type:
- Temperature:
Autosampler
- Type:
- Injection volume:
- Wash solvent:
Mass spectrometer
- Type:
- Ionization mode:
- Ionization voltage:
- Source temperature(s):
- Collision gas:
- Collision gas pressure:
- MS/MS-conditions:
- multiple reaction monitoring table
Analyte | Precursor [m/z] | Precursor [m/z] | Collision energy [eV] |
---|---|---|---|
species 1 | m/z 1 | m/z 2 | x |
species 2 | m/z 1 | m/z 2 | x |
Data analysis and quantification
Data handling
- chromatogram, spectrum and peak processing
Isotope correction
- if necessary
Calibration and quantification
- calibration type: matrix or external calibration by standard addition to sample matrix
- species used for calibration
Species | Material 1 | Material 2 |
---|---|---|
Species 1 | range pmol | range pmol |
Species 1 | range pmol | range pmol |