Lipid extraction according to Folch
From LipidomicsWiki
(Difference between revisions)
(New page: *Homogenize tissue in 2ml H<sub>2</sub>O / MeOH 1:1 *Add internal standard *Add 4ml CHCl<sub>3</sub> / MeOH 5:1 *Voretex and centrifuge for 10min at RT *Remove lower organic layer carefull...) |
Current revision (14:52, 10 July 2009) (view source) |
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| - | *Homogenize tissue in 2ml H<sub>2</sub>O / MeOH 1:1 | + | *Homogenize tissue in 2ml H<sub>2</sub>O / MeOH 1:1 |
| - | *Add internal standard | + | *Add internal standard |
| - | *Add 4ml CHCl<sub>3</sub> / MeOH 5:1 | + | *Add 4ml CHCl<sub>3</sub> / MeOH 5:1 |
| - | *Voretex and centrifuge for 10min at RT | + | *Voretex and centrifuge for 10min at RT |
| - | *Remove lower organic layer carefully into a smal vial | + | *Remove lower organic layer carefully into a smal vial |
| - | *Dry lower phase in a gentle stream of N<sub>2</sub> | + | *Dry lower phase in a gentle stream of N<sub>2</sub> |
*Resuspend in 200ul of CHCl<sub>3</sub> / MeOH 1:1 | *Resuspend in 200ul of CHCl<sub>3</sub> / MeOH 1:1 | ||
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| + | [[Category:Lipid_extraction]] | ||
Current revision
- Homogenize tissue in 2ml H2O / MeOH 1:1
- Add internal standard
- Add 4ml CHCl3 / MeOH 5:1
- Voretex and centrifuge for 10min at RT
- Remove lower organic layer carefully into a smal vial
- Dry lower phase in a gentle stream of N2
- Resuspend in 200ul of CHCl3 / MeOH 1:1
