Butanolic lipid extraction

From LipidomicsWiki

Description

• liquid-liquid extraction using butanol

Protocol

• 75 µl plasma were used for extraction
• to 75 µL plasma, add 20µL IS mix and 400µL phosphate buffer (containing 30mM citric acid and 40mM Na2HPO4)
• after short vortexing, add 1mL 1-butanol and 500µL water saturated 1-butanol
• after mixing for 15s the sample is centrifuged at high speed for 10 min
• the upper butanol phase containing the lipids is isolated, evaporated to dryness and redissolved in 200µL ethanol.

Reference

• Baker DL, Desiderio DM, Miller DD, Tolley B, Tiggyi GJ, Anal Biochem 2001, 292(2), 287-95