Butanolic lipid extraction

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Current revision (18:17, 28 January 2009) (view source)
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== Reference  ==
== Reference  ==
*[http://www.ncbi.nlm.nih.gov/pubmed/11355863?dopt=Citation Baker DL, Desiderio DM, Miller DD, Tolley B, Tiggyi GJ, Anal Biochem 2001, 292(2), 287-95]

Current revision


  • liquid-liquid extraction using butanol


  • 75 µl plasma were used for extraction
  • to 75 µL plasma, add 20µL IS mix and 400µL phosphate buffer (containing 30mM citric acid and 40mM Na2HPO4)
  • after short vortexing, add 1mL 1-butanol and 500µL water saturated 1-butanol
  • after mixing for 15s the sample is centrifuged at high speed for 10 min
  • the upper butanol phase containing the lipids is isolated, evaporated to dryness and redissolved in 200µL ethanol.


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