Butanolic lipid extraction
From LipidomicsWiki
(Difference between revisions)
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*after short vortexing, add 1mL 1-butanol and 500µL water saturated 1-butanol | *after short vortexing, add 1mL 1-butanol and 500µL water saturated 1-butanol | ||
*after mixing for 15s the sample is centrifuged at high speed for 10 min | *after mixing for 15s the sample is centrifuged at high speed for 10 min | ||
| - | *the upper | + | *the upper butanol phase containing the lipids is isolated, evaporated to dryness and redissolved in 200µL ethanol. |
== Reference == | == Reference == | ||
Revision as of 18:10, 28 January 2009
Description
- liquid-liquid extraction using butanol
Protocol
- 75 µl plasma were used for extraction
- to 75 µL plasma, add 20µL IS mix and 400µL phosphate buffer (containing 30mM citric acid and 40mM Na2HPO4)
- after short vortexing, add 1mL 1-butanol and 500µL water saturated 1-butanol
- after mixing for 15s the sample is centrifuged at high speed for 10 min
- the upper butanol phase containing the lipids is isolated, evaporated to dryness and redissolved in 200µL ethanol.
