Butanolic lipid extraction

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	== Reference  ==		== Reference  ==
-	*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-45BC6Y8-99&_user=616165&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000032338&_version=1&_urlVersion=0&_userid=616165&md5=864aa65a64c60e269861b38a1a4f3a74]	+	*[http://www.ncbi.nlm.nih.gov/pubmed/11355863?dopt=Citation Baker DL, Desiderio DM, Miller DD, Tolley B, Tiggyi GJ, Anal Biochem 2001, 292(2), 287-95]
	[[Category:Lipid_extraction]]		[[Category:Lipid_extraction]]

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Current revision

Description

• liquid-liquid extraction using butanol

Protocol

• 75 µl plasma were used for extraction
• to 75 µL plasma, add 20µL IS mix and 400µL phosphate buffer (containing 30mM citric acid and 40mM Na2HPO4)
• after short vortexing, add 1mL 1-butanol and 500µL water saturated 1-butanol
• after mixing for 15s the sample is centrifuged at high speed for 10 min
• the upper butanol phase containing the lipids is isolated, evaporated to dryness and redissolved in 200µL ethanol.

Reference

• Baker DL, Desiderio DM, Miller DD, Tolley B, Tiggyi GJ, Anal Biochem 2001, 292(2), 287-95