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HF diet for mouse feedingHMG-CoA reductase assayHNF
HTP Image analysis of LD size in differentiated adipocytesHUVEC isolation and cloning (JUMC)
Handling Synopsis Lists
Haschke et al.Hepatocyte and Stellate cell isolation from mouse liver
High-Content Imaging SPPsHigh pressure freezing and freeze substitution of cells grown on Aclar discs (Burger lab)
Hybridization of labeled RNA to cDNA Microarrays
Hydroxy/hydroperoxyeicosatetraenoic acids (FA0306)Images acquisition and analysis from De Matteis laboratory
Imaging of lipid droplets, nuclei and cytoplasm in the 96-well format (Thiele lab)Immunofluorescence and Bodipy staining of lipid dropletsImplementation of High-Content Imaging SPPs
Implementation of Mass Spectrometry of Lipids SPPsImplementation of Microarray SPPsImplementation of Proteomics SPPs
Implementation of Standard Processing ProceduresImplementation of TaqMan SPPsIn vivo cell monitoring - BD Pathway Bioimager 855 (JUMC)
Inositol phosphatesIsolation of lipid droplets from mouse primary hepatocytes
Isolation of primary hepatocytes from mouse liverIsoprenoids/RetinoidsIsoprostanes
Kerstin Leuthaeuser-Jaschinski
Kick off meeting - June 2008LC-MS
LXRLeukocyte populations and platelets
Leukotrienes (FA0302)
LinksLipases and LCAT
Lipid ClassLipid Conference CalendarLipid Storage Genes
Lipid extractionLipid extraction according to Bligh and Dyer
Lipid extraction according to FolchLipid extraction according to modified FolchLipid extraction for TLC (Chloroform-Methanol, Thiele lab)
Lipid extraction from yeastLipid extraction using ButanolLipid extraction using MTBE
Lipid mass spectrometric methods established in LipidomicNetLipid mass spectrometry
Lipid mass spectrometry SOPs deliverable statusLipid mass spectrometry equipment in LipidomicNet
Lipid microdomainsLipid probesLipid standards in the LipidomicNet
Lipid standards needed in LipidomicNetLipid standards used in LipidomicNet - Glycerophospholipids
Lipid standards used in LipidomicNet - SphingolipidsLipid standards used in LipidomicNet - SterolsLipidomicNet-ENOR joint workshop
LipidomicNet-ENOR workshop programLipidomicNet Final Meeting - 10/2012LipidomicNet Library
LipidomicNet publicationsLipidomics technologiesLipoprotein analysis by FPLC - ESI-MS/MS - Wiesner et al.
Lipoprotein preparationLipoprotein separation by FPLC - Wiesner et al.Lipoprotein sphingolipids as potential atherogenic factors
Lipoxins (FA0304)Lysophosphatidylcholine - ESI-MS/MS - Liebisch et al.
LysophospholipidsLysosomal lipid-binding proteins: purification
Lysosomal storage disordersMT 01.10.2008MT 02.07.2008
MT 03.03.2010MT 03.09.2008MT 03.12.2008
MT 05.11.2008MT 06.08.2008MT 08.10.2008
MT 09.07.2008MT 10.09.2008MT 10.11.2008
MT 10.12.2008MT 13.08.2008MT 14.01.2009
MT 15.06.2008MT 15.10.2008MT 16.07.2008
MT 17.09.2008MT 17.12.2008MT 19.11.2008
MT 20.08.2008MT 21.01.2009MT 22.10.2008
MT 23.07.2008MT 24.09.2008MT 24.12.2008
MT 25.06.2008MT 26.11.2008MT 27.08.2008
MT 29.10.2008MT 30.07.2008Magnetic bead isolation of Mitochondria and following western blot analysis
Main Page
Mass Spectrometry of Lipids SPPs
Mass spectrometry
Mechanisms of bile acid feedback inhibition of gene transcriptionMeeting Plan
Megakaryocyte Platelet SynopsisMembranesMetabolic diseases
Metabolism and subcellular compartmentalization of sphingolipids
Microarray SPPsMicroarraysMonoacylglycerophospholipids
Monocyte IsolationMonoradylglycerolsMouse Key Nodes
NetherlandsNeutral glycosphingolipids (SP05)
No AccessNomenclature of lipid species
Non targeted Lipid Profiling LC-FT-MS/MSNuclear receptor regulation of bile acid synthesisNuclear receptors
Offline nanoESI Phospholipid Profiling - LC-MS/MSOther hydroxylase pathwaysOxidized glycerophospholipids (GP20)
Oxysterol-binding proteinsOxysterol binding assayOxysterols
P28 JKUPPARPRP-platelet rich plasma
Phosphatidylcholine / Sphingomyelin - ESI-MS/MS - Liebisch et al.
Phosphatidylethanolamine-based plasmalogens - ESI-MS/MS - Liebisch et al.
Phosphatidylethanolamine - ESI-MS/MS - Liebisch et al.
Phosphatidylinositol metabolism
Phosphatidylserine - ESI-MS/MS - Liebisch et al.Phospholipid and neutral lipid droplet stainingPhospholipid class-specific profiling by LC/MS (Utrecht protocol)
Phospholipid metabolismPhospholipidosis detectionPhosphosphingolipids (SP03)
Plant sterolsPlatelet isolationPolyketides
Prenol LipidsPrenylationPreparation of delipidated FCS (Thiele lab)
Preprocessing and differential expression with CARMAWebPreprocessing of Single Colored Agilent ArraysPress releases
Processing Pipeline for High-Content Imaging SPPsProcessing Pipeline for Mass Spectrometry of Lipids SPPsProcessing Pipeline for Microarray SPPs
Processing Pipeline for Proteomics SPPsProcessing Pipeline for TaqMan SPPsProcessing Pipelines
Profiling of Neutral Lipids (Utrecht protocol)Profiling of Phospholipids (Utrecht protocol)Project Management
Project Status Report for Period 1Project Status Report for Period 2
Proposal for lipid MS nomenclatureProposal on lipid standards to be synthesizedProstaglandin quantification - GC-MS
Prostaglandins (FA0301)Proteomics SPPs
Protocols for stable isotope labelingPurification LDs from mouse adipocytesPurification LDs from mouse hepatocytes
Quinones and hydroquinonesRORRT-PCR SPPs
Real time interaction between immobilized liposomes and lipid binding proteins by Surface Plasmon ResonanceRegensburg2014Regensburg Chip Hybridization Center
ResourcesRetinoid determination - Gunderson et al.
Retinoid determination - Kane et al.Retinoid determination - Wang et al.Roche SPPs
SHP-dependent mechanismSHP-independent mechanismsSVF human cells isolation and culture (JUMC)
Schwedhelm et al.Secosteroids (ST03)
Setup of a lipid species databaseShorthand notation - General rulesShorthand notation for Fatty Acyls (FA)
Shorthand notation for Glycerolipids (GL)Shorthand notation for Glycerophospholipids (GP)Shorthand notation for Sphingolipids (SP)
Shorthand notation for Sterols (ST)Shorthand notation for lipid structures derived from mass spectrometrySiRNA knock down - A431 cells - 24 well plate - analysis by fluorescence microscopy (Thiele lab)
SiRNA knock down - A431 cells - 24 well plate - biochemical analysis (Thiele lab)SiRNA knock down - HeLa cells - 24 well plate - analysis by fluorescence microscopy (Thiele lab)SiRNA knock down - HeLa cells - 24 well plate - biochemical analysis (Thiele lab)
SiRNA knock down - HuH7 cells - 24 well plate - analysis by fluorescence microscopy (Thiele lab)SiRNA knock down - HuH7 cells - 24 well plate - biochemical analysis (Thiele lab)Signalling pathways induced by sphingolipids
Sircar et al.Site MapSkin lipid analysis - HPTLC - Farwanah et al.
Sphingoid bases (SP01)Sphingolipid analysis by mass spectrometry
Sphingolipid profiling - LC-MS/MS - Scherer et al.Sphingolipids (SP)
Sphingolipids and apoptosisSphingolipids and cell differentiationSphingolipids and cell migration and adhesion
Sphingolipids and vascular tone regulationSphingosine-1-phosphate, Lysophosphatidic acid - LC-MS/MS - Scherer et al.
Sphingosine-1-phosphate - LC-MS/MS - Berdyshev et al.Sphingosine-1-phosphate - LC-MS/MS - Bielawski et al.Sphingosine-1-phosphate - LC-MS/MS - Schmidt et al.
Sphingosine-1-phosphate quantitationSphingosine/Sphinganine - LC-MS/MS - Lieser et al.
Staining of cellsStaining of lipid droplets with Nile red and BODIPY 493/503Staining of lipid droplets with oil Red O
Standard Processing ProceduresSteering Committee Meeting - 01/2010Steering Committee Meeting - 01/2011
Steering Committee Meeting - 01/2012Steering Committee Meeting - 07/2009Steering Committee Meeting - 07/2010
Steering Committee Meeting - 11/2008Steering Committee Meeting 2011/06
Steroid conjugates (ST05)Steroid hormones
Steroids (ST02)Sterol analysis by mass spectrometrySterols
Sterols (ST)Sterols (ST) determination - Lembcke et al.Sterols (ST) determination - Mezine et al.
Sterols (ST01)Subcategory: MonoacylglycerophospholipidsSynthesis and metabolism of straight chain fatty acids
Synthesis of PS and formation of PE in the mitochondrial PSDecarboxylation pathway
TF IV 02.09.2008TF IV 05.08.2008
TF IV 09.12.2008TF IV 11.11.2008TF IV 14.10.2008
TF IV 16.09.2008TF IV 19.08.2008TF IV 20.01.2009
TF IV 23.12.2008TF IV 25.11.2008TF IV 28.10.2008
TF IV 30.09.2008TF I 21.10.2008
TF VIII 03.12.2008TF VIII 08.10.2008TF VIII 13.08.2008
TF VIII 19.11.2008TF VIII 22.04.2009TF VIII 22.10.2008
TF VIII 28.01.2009TF VIII 30.07.2008TF VIII ActionPoints 2010
TF VII 01.10.2008TF VII 03.09.2008
TF VII 06.08.2008TF VII 10.12.2008TF VII 12.11.2008
TF VII 15.10.2008TF VII 17.09.2008
TF VII 20.08.2008TF VII 24.12.2008TF VII 26.11.2008
TF VII 29.10.2008TIC 2013 Tbilisi International Conference 2013Tagged constructs of lipid droplet proteins for imaging
TaqMan Arrays
TaqMan SPPsTask Force ITask Force II
Task Force IIITask Force IVTask Force IX
Task Force I - internal pagesTask Force OverviewTask Force V
Task Force VITask Force VIITask Force VIII
Task Force VIII phone conferencesTemplates
Templates for new articlesTemplates for presentationsTeststartpage
Teunissen et al.The classic bile acid biosynthetic (neutral) pathwayThe “Kennedy pathway” as the majour route of PC and PE synthesis
Thin layer chromatography (Thiele Lab)Thromboxanes (FA0303)
ToolsTotal Lipid Profiling LTQ-FTTraining
Transferproteins – CETP, PLTPTriglyceride hydrolysis kineticsUK
Uptake of glycerophospholipid precursors and the interconversion of glycerophospholipid speciesVisualization of fixed/permeabilized cultured cells (JUMC)
WP7WP8WP III TF V 05.09.2008
WP III TF V 14.10.2008WP III TF V 25.11.2008
WebexWestern blot of membrane proteinsWiki Roadmap
Work Package Overview
Workshop - 2008/09Workshop - 2008/09 - presentations
Workshop - 2009/03Workshop - 2009/10Workshop - 2009/10 - Abstracts
Workshop - 2010/04Workshop - 2011/05Workshop - 2011/10
Workshop - 2012/07

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