Lipoprotein separation by FPLC - Wiesner et al.

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Contents

Instrument

  • Type: Pharmacia Smart System® including fraction collector
  • Running buffer: Dulbecco's PBS containing 1mM EDTA
  • Flow: constant flow of 40μL/min


Column

  • Type: Superose 6 PC 3.2/30 column (GE Healthcare)
  • Temperature: room temperature


Fractionation

  • Injection volume: 50 µL human serum
  • Fractionation start: 18min
  • Fraction volume: 80µl
  • Number of fractions: 20


Example

Analysis of cholesterol and triglyceride content of the fractions of a human normolipidemic serum.



Reference

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