Lipid extraction according to Folch

From LipidomicsWiki

Jump to: navigation, search
  • Homogenize tissue in 2ml H2O / MeOH 1:1
  • Add internal standard
  • Add 4ml CHCl3 / MeOH 5:1
  • Voretex and centrifuge for 10min at RT
  • Remove lower organic layer carefully into a smal vial
  • Dry lower phase in a gentle stream of N2
  • Resuspend in 200ul of CHCl3 / MeOH 1:1
Retrieved from "https://www.lipidomicnet.org/index.php/Lipid_extraction_according_to_Folch"
Personal tools
Create a book