Cell culture from DeMatteis lab
From LipidomicsWiki
| Cell line | Tissue, Species | Culturing media | Freezing media | Transfection | Group |
|---|---|---|---|---|---|
| HeLa | Adenocarcinoma cervix, Epithelial,Human | DMEM 1x, 10% Fetal calf serum (FCS), 1% Pen/Strep, 1% Glutamine | 10%DMSO in FCS |
TransIT LT1 (DNA), Oligofectamine(siRNAs) | De Matteis |
| Cos7 | SV40-transformed Kidney, fibroblast,monkey | DMEM 1x, 10% FCS, 1% Pen/Strep, 1% Glutamine | 10%DMSO in FCS |
TransFast, Electroporation 220V 950mFa (DNA) Oligofectamine(siRNAs) | De Matteis |
| HK-2 | HPV16-transformed Kidney proximal tubule, Epithelial,Human | DMEM NUT MIX F12 1x, 5%FCS 1% Pen/Strep, Insulin 5mg/ml /Transferrin 5mg/ml /Selenium 5ng/ml | DMEM 7,5% DMSO 5%FCS | TransIT LT1, Electroporation 380V 850mFa (DNA) Dharmafect 4(siRNAs) | De Matteis |
| NRK | Normal Kidney, Fibroblast,Rat | DMEM 1x, 5% FCS, 1% Pen/Strep, 1% Glutamine, 1% Non-essential aminoacids (NEAA) | 10%DMSO in FCS |
Electroporation 250 V 975 mFa (DNA) Lipofectamine2000 (siRNAs) | De Matteis |
| HepG2 | Hepatocellular carcinoma, Epithelial,Human | MEM 1x, 10%FCS, 1% Pen/Strep, 1% Glutamine, 1% Non-essential aminoacids (NEAA), Sodium Piruvate | 10%DMSO in FCS | Dharmafect 4(siRNAs) | De Matteis |
