Butanolic lipid extraction

From LipidomicsWiki

Jump to: navigation, search


  • liquid-liquid extraction using butanol


  • 75 µl plasma were used for extraction
  • to 75 µL plasma, add 20µL IS mix and 400µL phosphate buffer (containing 30mM citric acid and 40mM Na2HPO4)
  • after short vortexing, add 1mL 1-butanol and 500µL water saturated 1-butanol
  • after mixing for 15s the sample is centrifuged at high speed for 10 min
  • the upper butanol phase containing the lipids is isolated, evaporated to dryness and redissolved in 200µL ethanol.


Personal tools
Create a book