From LipidomicsWiki
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- 17:29, 10 November 2008 (hist) (diff) Task Force V
- 17:24, 10 November 2008 (hist) (diff) User:Christian Wolfrum (top)
- 17:21, 10 November 2008 (hist) (diff) N Image:Wolfrum.jpg (top)
- 17:18, 10 November 2008 (hist) (diff) N Image:Christian Wolfrum.jpg (top)
- 17:12, 10 November 2008 (hist) (diff) N User:Christian Wolfrum (New page: {{ Infobox_User |myImage= |myInstitution= ETH Zürich |myCountry= Switzerland |myTitle= Prof. Dr. |myExpertise= Adipocyte Development |myAbout= |myTaskforces= TF IV, V |myWorkpackage= WP 3...)
- 09:07, 15 October 2008 (hist) (diff) N WP III TF V 14.10.2008 (New page: Minutes WP3 & TF V Meeting présent : Paris : Dugail , Le Lay <br> Zürich : Wolfrum <br> Leiden : van Dyk <br> Utrecht : Houwelig, Helms <br> Italien : Moschetta<br> Graz : Spener ...)
- 16:58, 14 October 2008 (hist) (diff) N HTP Image analysis of LD size in differentiated adipocytes (New page: General: <br>use black plates (384 or 96 well plates)<br> This protocol is designed for fixed cells; all dyes can be adapted for live cells <br> Fixation:<br>Remove culture medium<br>Was...)
- 16:53, 14 October 2008 (hist) (diff) N Purification LDs from mouse hepatocytes (New page: Isolation of hepatocytes Solutions:<br>Buffer 1: 1 L Krebs-Henseleit buffer (KHB) (NaCl 115 mmol/l; NaHCO3 25 mmol/l; KCl 5,9 mmol/l; MgCl2 1,18 mmol/l; NaH2PO4 1,23 mmol/l; Na2SO4 1,2 mm...)
- 16:52, 14 October 2008 (hist) (diff) N Purification LDs from mouse adipocytes (New page: Purification of lipid droplets from adipocytes 1. Resuspend cells in disruption buffer containing protease inhibitors and 1 mM EDTA. 2. Disrupt cells with a nitrogen bomb or by sonicatio...)
- 16:50, 14 October 2008 (hist) (diff) N Hepatocyte and Stellate cell isolation from mouse liver (New page: Hepatocyte and Stellate cell Isolation (SOP) Procedure according to P.O. Seglen, Meth. Cell Biol. 13 (1976) 29-83 adapted by L. Riccalton-Banks et al. Mol. Cell. Biochemistry 248 (2003),...)
- 16:44, 14 October 2008 (hist) (diff) N HF diet for mouse feeding
- 16:37, 14 October 2008 (hist) (diff) N Adipocyte and SVF isolation from mouse adipose tissue (New page: SV-fraction/Adipocyte Isolation <br>Solutions :<br>- medium: DMEM (+ pyr + gln, 4,5 g/l gluc), 10 % FBS, Pen/Strep<br>- collagenase buffer:<br>NaHCO3 25 mM 84,01 g/mol 1,05 g<br>KH2PO4 12 ...)
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