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1st Annual LipidomicNet Steering intern1st Annual Steering Committee Meeting
ABC transporterACAT in vitro assay
Acid ceramidase assayAcidic glycosphingolipids (SP06)AdditionalGroups
Adipocyte and SVF isolation from mouse adipose tissueAdipocyte isolation and cultivation
Affymetrix SPP
Agilent Feature ExtractionAgilent not mapped probes
Aminoallyl Labeling of RNA for Microarray HybridizationAnalysis of lipid metabolism kinetics by pulse-chase in living cells (Thiele lab)Antibodies against lipid droplet proteins
Antibodies from DeMatteis labApoA-I containing lipoproteinsApoB containing lipoproteins
Available DataBeneficiary
Bile acid quantitation - LC-MS/MSBile acids/-conjugates
Bile acids/-conjugates determination - Perwaiz et al.Bile acids/-conjugates determination - Tagliacozzi et al.Bile acids/-conjugates determination - Von Eckardstein
Bile acids - LC-MS/MS - Scherer et al.Bile acids and derivatives (ST04)BioUML
BlackboardBranched chain fatty acidsButanolic lipid extraction
CARCDP-Glycerols (GP13)Cardiovascular diseases
Cell culture from DeMatteis labCell isolation proceduresCell proliferation BrdU (JUMC)
Ceramide - ESI-MS/MS - Liebisch et al.Ceramide Profiling LC-MS/MS
Ceramide phosphocholines (sphingomyelins) (SP0301)Ceramides (SP02)
Ceramides - stratum corneum, free extractable - LC-MS - Farwanah et al.Cholesterol
Cholesterol/sterol analysis - GCMSCholesterol / Cholesterylester - ESI-MS/MS - Liebisch et al.
Cholesterol analysis - GCMSCholesterol biosynthesis assayCholesterol sulfate
CholesteroylationCholesteryl ester synthesis assayCurrent events
Cytotoxicity assay (JUMC)DNA constructs from DeMatteis labData Management Training Workshop Cambridge 2010
Data type taskforcesData types and attributes in lipid species databases
DatabasesDavies et al.Degradation of FA (b-oxidation)
DeliverablesDescription of MS methods in databases
Description of samples in databasesDi-glycerol tetraether phospholipids (caldarchaeols) (GP18)Differential Expression using the B-Fabric Analysis tool
Differential Expression with ChipInspectorDiscussion on lipid standards in LipidomicNet
Discussion on the mass spectrometric analysis of triglyceride rich samplesDocosanoids (FA04)Document:From SDS to Integromics software.doc
EU Framework VII project "LipidomicNet"Eicosanoid determination - Harkewitz et al.
Eicosanoid determination - Masoodi et al.Eicosanoid determination - Yue et al.
Eicosanoids (FA03)Ensembl genes without Agilent Probe ID
Epoxyeicosatrienoic acids (FA0308)
Erythrocyte isolation
Ethics proposalsExtensions LS/myCSS.cssFAQ
FAS IIFATPFA uptake and transport proteins
FXRFatty Acid Profiling GC-MS
Fatty Acid analysis - GCMS
Fatty Acids and Conjugates (FA01)Fatty Acyls (FA)Fatty Acyls analysis by mass spectrometry
Fatty acid oxidative conversionFatty acid remodellingFilipin staining of cells
First Bioinformatics Workshop, Sept 2008First Bioinformatics Workshop - presentations
Fixation and permeabilization protocols from DeMAtteis labFixation of cells with 4% paraformaldehydeFixed/permeabilized cultured cells - BD Pathway Bioimager 855 (JUMC)
Flow cytometryFluorescence Microscopy (High-Content Imaging) SPP for the Evotec Opera automated microscopeFluorescence Microscopy (High-Content Imaging) SPP for the analysis of lipid droplets for the Discovery-1 automated microscope of Molecular Devices
Fluorescent microscopy of neutral lipids in yeastFree cholesterol/cholesterol ester distributionFreeze Fracture
GC-MSGanglioside extractionGlycerol-nonitol tetraether phospholipids (GP19)
Glycerolipid Profiling - FT-MSGlycerolipids (GL)
Glycerophosphates (GP10)Glycerophosphatidylinositol (GPI)-anchorGlycerophosphocholines (GP01)
Glycerophosphoethanolamines (GP02)Glycerophosphoglycerols (GP04)Glycerophosphoglycerophosphates (GP05)
Glycerophosphoglycerophosphoglycerols (GP12)Glycerophosphoinositolglycans (GP15)Glycerophosphoinositols (GP06) / phosphates (GP07-09)
Glycerophospholipid Profiling - FT-MSGlycerophospholipid Profiling - LC-MS/MS
Glycerophospholipids (GP)Glycerophospholipids analysis by mass spectrometry
Glycerophosphonocholines (GP16)Glycerophosphonoethanolamines (GP17)Glycerophosphoserines (GP03)
Glyceropyrophosphates (GP11)GlycosphingolipidsGlycosylglycerophospholipids (GP14)
Granulocyte-enrichmentGranulocyte isolation
HF diet for mouse feedingHMG-CoA reductase assay
HNFHTP Image analysis of LD size in differentiated adipocytes
HUVEC isolation and cloning (JUMC)Handling Synopsis Lists
Haschke et al.
Hepatocyte and Stellate cell isolation from mouse liverHigh-Content Imaging SPPsHigh pressure freezing and freeze substitution of cells grown on Aclar discs (Burger lab)
High throughput workshop - March 2009
Hybridization of labeled RNA to cDNA MicroarraysHydroxy/hydroperoxyeicosatetraenoic acids (FA0306)
Images acquisition and analysis from De Matteis laboratoryImaging of lipid droplets, nuclei and cytoplasm in the 96-well format (Thiele lab)Immunofluorescence and Bodipy staining of lipid droplets
Implementation of High-Content Imaging SPPsImplementation of Mass Spectrometry of Lipids SPPsImplementation of Microarray SPPs
Implementation of Proteomics SPPsImplementation of Standard Processing ProceduresImplementation of TaqMan SPPs
In vivo cell monitoring - BD Pathway Bioimager 855 (JUMC)Inositol phosphatesInternational lipid meetings
Isolation of lipid droplets from mouse primary hepatocytesIsolation of primary hepatocytes from mouse liverIsoprenoids/Retinoids
Isoprostanes
Kerstin Leuthaeuser-JaschinskiKick off meeting - June 2008
LC-MSLXR
Leukocyte populations and plateletsLeukotrienes (FA0302)
Links
Lipases and LCATLipid ClassLipid extraction
Lipid extraction according to Bligh and DyerLipid extraction according to Folch
Lipid extraction according to modified FolchLipid extraction for TLC (Chloroform-Methanol, Thiele lab)Lipid extraction from yeast
Lipid extraction using ButanolLipid extraction using MTBELipid mass spectrometric methods established in LipidomicNet
Lipid mass spectrometry
Lipid mass spectrometry SOPs deliverable statusLipid mass spectrometry equipment in LipidomicNetLipid microdomains
Lipid probesLipid standards in the LipidomicNetLipid standards needed in LipidomicNet
Lipid standards used in LipidomicNet - GlycerophospholipidsLipid standards used in LipidomicNet - Sphingolipids
Lipid standards used in LipidomicNet - SterolsLipidomicNet publicationsLipidomics technologies
Lipoprotein analysis by FPLC - ESI-MS/MS - Wiesner et al.Lipoprotein preparationLipoprotein separation by FPLC - Wiesner et al.
Lipoxins (FA0304)Lysophosphatidylcholine - ESI-MS/MS - Liebisch et al.
LysophospholipidsLysosomal lipid-binding proteins: purification
MT 01.10.2008MT 02.07.2008MT 03.03.2010
MT 03.09.2008MT 03.12.2008MT 05.11.2008
MT 06.08.2008MT 08.10.2008MT 09.07.2008
MT 10.09.2008MT 10.11.2008MT 10.12.2008
MT 13.08.2008MT 14.01.2009MT 15.06.2008
MT 15.10.2008MT 16.07.2008MT 17.09.2008
MT 17.12.2008MT 19.11.2008MT 20.08.2008
MT 21.01.2009MT 22.10.2008MT 23.07.2008
MT 24.09.2008MT 24.12.2008MT 25.06.2008
MT 26.11.2008MT 27.08.2008MT 29.10.2008
MT 30.07.2008Magnetic bead isolation of Mitochondria and following western blot analysisMain Page
Mass Spectrometry of Lipids SPPs
Mass spectrometryMeeting Plan
Megakaryocyte Platelet SynopsisMembranes
Metabolic diseases
Microarray SPPsMicroarraysMonoacylglycerophospholipids
Monocyte IsolationMonoradylglycerolsNeutral glycosphingolipids (SP05)
No Access
Non targeted Lipid Profiling LC-FT-MS/MSOffline nanoESI Phospholipid Profiling - LC-MS/MSOxidized glycerophospholipids (GP20)
Oxysterol-binding proteinsOxysterol binding assayOxysterols
P01 UHREGP02 UUP03 CMNS
P04 INSERMP05 LUMCP06 ETHZ
P07 UNIGRAZP08 UGOTP09 JUMC
P10 MPGP11 BRC HASP12 IEE SAS
P13 BABRAHAMP14 UHP15 UBO
P16 EMBLP17 UMG GOEP18 BIOBASE
P19 ZORAP20 PROTAGENP21 INTEGROMICS
P22 UOXF.H7P23 MRCP24 JKU
P25 ISBP26 WWUPPAR
PRP-platelet rich plasmaPXR/SXRPalmitoylation
Phosphatidylcholine / Sphingomyelin - ESI-MS/MS - Liebisch et al.
Phosphatidylethanolamine-based plasmalogens - ESI-MS/MS - Liebisch et al.Phosphatidylethanolamine - ESI-MS/MS - Liebisch et al.
Phosphatidylserine - ESI-MS/MS - Liebisch et al.
Phospholipid and neutral lipid droplet stainingPhospholipid class-specific profiling by LC/MS (Utrecht protocol)Phospholipidosis detection
Phosphosphingolipids (SP03)Plant sterolsPlatelet isolation
PolyketidesPrenol LipidsPrenylation
Preparation of delipidated FCS (Thiele lab)Preprocessing and differential expression with CARMAWebPreprocessing of Single Colored Agilent Arrays
Press releasesProcessing Pipeline for High-Content Imaging SPPsProcessing Pipeline for Mass Spectrometry of Lipids SPPs
Processing Pipeline for Microarray SPPsProcessing Pipeline for Proteomics SPPsProcessing Pipeline for TaqMan SPPs
Processing PipelinesProfiling of Neutral Lipids (Utrecht protocol)Profiling of Phospholipids (Utrecht protocol)
Project Status Report for Period 1Project Status Report for Period 2Proposal for lipid MS nomenclature
Proposal on lipid standards to be synthesizedProstaglandin quantification - GC-MS
Prostaglandins (FA0301)Proteomics SPPsProtocols for stable isotope labeling
ProtokollPublications resulting from LipidomicNetPurification LDs from mouse adipocytes
Purification LDs from mouse hepatocytesQuinones and hydroquinonesROR
RT-PCR SPPsReal time interaction between immobilized liposomes and lipid binding proteins by Surface Plasmon ResonanceRegensburg Chip Hybridization Center
Retinoid determination - Gunderson et al.Retinoid determination - Kane et al.
Retinoid determination - Wang et al.Roche SPPsSVF human cells isolation and culture (JUMC)
Saccharolipids
Schwedhelm et al.Secosteroids (ST03)
Setup of a lipid species databaseSiRNA knock down - A431 cells - 24 well plate - analysis by fluorescence microscopy (Thiele lab)SiRNA knock down - A431 cells - 24 well plate - biochemical analysis (Thiele lab)
SiRNA knock down - HeLa cells - 24 well plate - analysis by fluorescence microscopy (Thiele lab)SiRNA knock down - HeLa cells - 24 well plate - biochemical analysis (Thiele lab)SiRNA knock down - HuH7 cells - 24 well plate - analysis by fluorescence microscopy (Thiele lab)
SiRNA knock down - HuH7 cells - 24 well plate - biochemical analysis (Thiele lab)Sircar et al.Skin lipid analysis - HPTLC - Farwanah et al.
Sphingoid bases (SP01)Sphingolipid analysis by mass spectrometry
Sphingolipid profiling - LC-MS/MS - Scherer et al.Sphingolipids (SP)
Sphingosine-1-phosphate, Lysophosphatidic acid - LC-MS/MS - Scherer et al.Sphingosine-1-phosphate - LC-MS/MS - Berdyshev et al.
Sphingosine-1-phosphate - LC-MS/MS - Bielawski et al.Sphingosine-1-phosphate - LC-MS/MS - Schmidt et al.
Sphingosine-1-phosphate quantitation
Sphingosine/Sphinganine - LC-MS/MS - Lieser et al.Staining of cells
Staining of lipid droplets with Nile red and BODIPY 493/503Staining of lipid droplets with oil Red OStandard Processing Procedures
Steering Committee Meeting Munich 2010Steering Committee Meeting Regensburg, November 2008
Steering Committee Review Meeting Zurich, July 5-6, 2010Steroid conjugates (ST05)
Steroid hormonesSteroids (ST02)Sterol analysis by mass spectrometry
SterolsSterols (ST)Sterols (ST) determination - Lembcke et al.
Sterols (ST) determination - Mezine et al.Sterols (ST01)Subcategory: Monoacylglycerophospholipids
TF8 ActionPoints2010
TF IV 02.09.2008TF IV 05.08.2008TF IV 09.12.2008
TF IV 11.11.2008TF IV 14.10.2008TF IV 16.09.2008
TF IV 19.08.2008TF IV 20.01.2009TF IV 23.12.2008
TF IV 25.11.2008TF IV 28.10.2008TF IV 30.09.2008
TF I 21.10.2008TF VIII 03.12.2008
TF VIII 08.10.2008TF VIII 13.08.2008TF VIII 19.11.2008
TF VIII 22.04.2009TF VIII 22.10.2008TF VIII 28.01.2009
TF VIII 30.07.2008TF VII 01.10.2008
TF VII 03.09.2008TF VII 06.08.2008TF VII 10.12.2008
TF VII 12.11.2008TF VII 15.10.2008TF VII 17.09.2008
TF VII 20.08.2008TF VII 24.12.2008
TF VII 26.11.2008TF VII 29.10.2008Tagged constructs of lipid droplet proteins for imaging
TaqMan Arrays
TaqMan SPPsTask Force ITask Force II
Task Force IIITask Force IVTask Force IX
Task Force I - internal pagesTask Force VTask Force VI
Task Force VIITask Force VIIITask Force VIII phone conferences
Task ForcesTemplatesTemplates for new articles
Templates for presentationsTeunissen et al.Thin layer chromatography (Thiele Lab)
Thromboxanes (FA0303)Transferproteins – CETP, PLTP
Triglyceride hydrolysis kineticsVisualization of fixed/permeabilized cultured cells (JUMC)
WP1WP2WP3
WP4WP5WP6
WP7WP8WP III TF V 05.09.2008
WP III TF V 14.10.2008WP III TF V 25.11.2008
WebexWestern blot of membrane proteins
Wikipedia:tutorialWorkshop Graz 2009-10-26 27
Workshop Graz 2009-10-26 27 Abstracts

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