RT-PCR SPPs

From LipidomicsWiki

Introduction

The RT-PCR processing workflow consists of several different steps. After an RT-PRC run is completed the cycle of quantitation (Cq) has to be determined, the efficiency of the PCR reaction hast to be calculated, the individual targets have to be normalized to one or more reference genes and differential expressed genes have to be determined by statistical analysis.

We have defined two SPPs: one for ABI Taqman assays and another one for ABI SYBRGreen and Roche Lightcycler assays.

SPPs for TAQMAN platform

• RT-PCR (ABI TaqMan) SPPs

SPPs for SYBRGreen & Roche LightCycler platforms

• RT-PCR (ABI SYBRGreen & Roche LightCycler) SPPs

Back to Standard Processing Procedures Main Page