Lipoprotein separation by FPLC - Wiesner et al.

From LipidomicsWiki

Contents 1 Instrument 2 Column 3 Fractionation 4 Example 5 Reference

Instrument

• Type: Pharmacia Smart System® including fraction collector
• Running buffer: Dulbecco's PBS containing 1mM EDTA
• Flow: constant flow of 40μL/min

Column

• Type: Superose 6 PC 3.2/30 column (GE Healthcare)
• Temperature: room temperature

Fractionation

• Injection volume: 50 µL human serum
• Fractionation start: 18min
• Fraction volume: 80µl
• Number of fractions: 20

Example

Analysis of cholesterol and triglyceride content of the fractions of a human normolipidemic serum.

Reference

• Wiesner et al. J Lipid Res. 2009 Mar;50(3):574-85.