Document:From SDS to Integromics software.doc

From LipidomicsWiki

How to export a RT-PCR study from SDS RQ Manager (AB platforms)

The process to make a RT-PCR study readable into Integromics software involves the following steps:

     1) Load your RT-PCR study from a SDS file (add as much plates as the study contains)
     2) Click on “Analyze all” button on the upper tool bar (thus the application software will calculate the corresponding Ct value for each gene from the fluorescence curves)
     3) Now it’s time to export the analysis results so set the “Table orientation” (in the upper tool bar) as “Plate Centric”
     4) Select all the plates that the study contains by clicking on each plate (keeping CTRL button pressed)
     5) Save the study in order to allow the export step
     6) Click on File, Export, Results Data
     7) Put a name to your output file and save it as a tab delimited txt file